Publications
Flagel SB, Robinson TE, & Sarter M. (2021). Comment on Pohorala et al.: Sign-tracking as a predictor of addiction vulnerability. Psychopharmacology, 238(9), 2661-2664. https://doi.org/10.1007/s00213-021-05927-3
Comments on an article by V. Pohorala et al. (see record 2021-43982-001). The authors examined the relationship between the propensity to attribute incentive salience to reward cues and addiction-like behavior using the 3-criteria model of drug self-administration adapted from and reflective of the criteria for addiction in the Diagnostic and Statistical Manual of Mental Disorders (DSMIV). Briefly, male rats were characterized as goal-trackers (GT), intermediate responders (INT), or signtrackers (ST), based on their Pavlovian conditioned approach (PCA) response to a lever-cue (conditioned stimulus) that predicted liquid reward delivery (unconditioned stimulus). Subsequently, rats underwent 43 daily sessions of cocaine self-administration and were assessed for (1) the persistence of drug-seeking, (2) motivation for cocaine-taking, and (3) resistance to punishment. Correlational analyses indicated no relationship between these three indices of addiction like behavior and Pavlovian conditioned approach behavior, although sign-trackers showed greater resistance to punishment relative to goal-trackers. The authors concluded that “behavior along the continuum of GT, INT, and ST PCA scores may not be a reliable predictor of the onset or severity of cocaine addiction…”, as captured by the 3-criteria model. The purpose of this commentary is to ofer further explanation of these findings regarding the nature of the susceptibility to addiction predicted by the sign-tracking phenotype.
Kuhn BN, Campus P, Klumpner MS, Chang SE, Iglesias AG, & Flagel SB. (2021). Inhibition of a cortico-thalamic circuit attenuates cue-induced reinstatement of drug-seeking behavior in 'relapse prone' male rats. Psychopharmacology (Berl). https://doi.org/10.1007/s00213-021-05894-9
Relapse often occurs when individuals are exposed to stimuli or cues previously associated with the drug-taking experience. The ability of drug cues to trigger relapse is believed to be a consequence of incentive salience attribution, a process by which the incentive value of reward is transferred to the reward-paired cue. Sign-tracker (ST) rats that attribute enhanced incentive value to reward cues are more prone to relapse compared to goal-tracker (GT) rats that primarily attribute predictive value to such cues.
Iglesias AG & Flagel SB. The paraventricular thalamus as a critical node of motivated behavior via the hypothalamic-thalamic-striatal circuit. Frontiers in Integrative Neuroscience, https://doi.org/10.3389/fnint.2021.706713
In this review, we highlight evidence that supports a role for the paraventricular nucleus of the thalamus (PVT) in motivated behavior. We include a neuroanatomical and neurochemical overview, outlining what is known of the cellular makeup of the region and its most prominent afferent and efferent connections. We discuss how these connections and distinctions across the anterior-posterior axis correspond to the perceived function of the PVT. We then focus on the hypothalamic-thalamic-striatal circuit and the neuroanatomical and functional placement of the PVT within this circuit. In this regard, the PVT is ideally positioned to integrate information regarding internal states and the external environment and translate it into motivated actions. Based on data that has emerged in recent years, including that from our laboratory, we posit that orexinergic (OX) innervation from the lateral hypothalamus (LH) to the PVT encodes the incentive motivational value of reward cues and thereby alters the signaling of the glutamatergic neurons projecting from the PVT to the shell of the nucleus accumbens (NAcSh). The PVT-NAcSh pathway then modulates dopamine activity and resultant cue-motivated behaviors. As we and others apply novel tools and approaches to studying the PVT we will continue to refine the anatomical, cellular, and functional definitions currently ascribed to this nucleus and further elucidate its role in motivated behaviors.
Krueger LD, Chang SE, Motoc M, Chojecki M, Freeman ZT, & Flagel SB. (2021). Effects of pair housing on patency of jugular catheters in rats (Rattus norvegicus). Journal of the American Association for Laboratory Animal Science, 60(3):357-364. https://doi.org/10.30802/AALAS-JAALAS-20-000071
Chronic vascular access devices are widely used in a variety of species for repeated blood sampling or substance administration. Jugular catheters are commonly used for studying addiction-related behaviors in rats. Rats with catheters have historically been individually housed for the duration of the study to prevent cage mates from damaging the catheter. The 2 goals of this study were to determine 1) the effects of pair housing on catheter patency and 2) the effects of pair housing on catheter patency of rats in a study of opioid self-administration and cue-induced reinstatement of opioid-seeking behavior. The latter study also represented an opportunity for experimental refinement as it evaluated the temporary use of a barrier that allowed for pair-housed rats to be physically separated. Male Heterogeneous Stock (HS; n = 24) and Sprague–Dawley (SD; n = 121) rats were allocated to either single- or pair-housed condition. To assess the effect of social housing on catheter patency, rats (HS, n = 24; SD, n = 36) were monitored in their assigned housing condition for one month, with scheduled evaluation of catheter patency and structural damage. To examine the effect of social housing on catheter patency during a study of opioid self-administration and cue-induced reinstatement of opioid-seeking behavior, rats (SD, n = 85) were monitored in their assigned housing condition with similar routine patency evaluations. Catheter patency rates between single- and pairhoused rats were not statistically different in the first experiment, and pair-housed animals were successfully maintained on an infusion study in the second experiment. The use of a barrier between pair-housed rats after surgery allowed continued social contact with no observed adverse effects. These results suggest that, pair housing is a viable option for rats with chronic vascular implants, and may improve their wellbeing by allowing them to display species-typical social behaviors.
Lopez SA, Mubarak E, Yang C, Parsegian A, Klumpner M, Campus P, & Flagel SB. (2021). Male goal-tracker and sign-tracker rats do not differ in neuroendocrine or behavioral measures of stress reactivity. eNeuro. 8 (3) ENEURO.0384-20.2021. https://doi.org/10.1523/ENEURO.0384-20.2021
Environmental cues attain the ability to guide behavior via learned associations. As predictors, cues can elicit adaptive behavior and lead to valuable resources (e.g., food). For some individuals, however, cues are transformed into incentive stimuli and elicit motivational states that can be maladaptive. The goal-tracker (GT)/sign-tracker (ST) animal model captures individual differences in cue-motivated behaviors, with reward-associated cues serving as predictors of reward for both phenotypes but becoming incentive stimuli to a greater degree for STs. While these distinct phenotypes are characterized based on Pavlovian conditioned approach (PavCA) behavior, they exhibit differences on a number of behaviors relevant to psychopathology. To further characterize the neurobehavioral endophenotype associated with individual differences in cue-reward learning, neuroendocrine and behavioral profiles associated with stress and anxiety were investigated in male GT, ST, and intermediate responder (IR) rats. It was revealed that baseline corticosterone (CORT) increases with Pavlovian learning, but to the same degree, regardless of phenotype. No significant differences in behavior were observed between GTs and STs during an elevated plus maze (EPM) or open field test (OFT), nor were there differences in CORT response to the OFT or physiological restraint. Upon examination of central markers associated with stress reactivity, we found that STs have greater glucocorticoid receptor (GR) mRNA expression in the ventral hippocampus, with no phenotypic differences in the dorsal hippocampus or prelimbic cortex (PrL). These findings demonstrate that GTs and STs do not differ on stress-related and anxiety-related behaviors, and suggest that differences in neuroendocrine measures between these phenotypes can be attributed to distinct cue-reward learning styles.
Lopez SA & Flagel SB. (2021). A proposed role for glucocorticoids in mediating dopamine-dependent cue-reward learning. Stress, 24(2):154-167. https://doi.org/10.1080/10253890.2020.1768240
Learning to respond appropriately to one’s surrounding environment is fundamental to survival. Importantly, however, individuals vary in how they respond to cues in the environment and this variation may be a key determinant of psychopathology. The ability of seemingly neutral cues to promote maladaptive behavior is a hallmark of several psychiatric disorders including, substance use disorder, post-traumatic stress disorder, eating disorders and obsessive-compulsive disorder. Thus, it is important to uncover the neural mechanisms by which such cues are able to attain inordinate control and promote psychopathological behavior. Here, we suggest that glucocorticoids play a critical role in this process. Glucocorticoids are primarily recognized as the main hormone secreted in response to stress but are known to exert their effects across the body and the brain, and to affect learning and memory, cognition and reward-related behaviors, among other things. Here we speculate that glucocorticoids act to facilitate a dopamine-dependent form of cue-reward learning that appears to be relevant to a number of psychiatric conditions. Specifically, we propose to utilize the sign-tracker/goal-tracker animal model as a means to capture individual variation in stimulus-reward learning and to isolate the role of glucocorticoid-dopamine interactions in mediating these individual differences. It is hoped that this framework will lead to the discovery of novel mechanisms that contribute to complex neuropsychiatric disorders and their comorbidity.
King CP, Tripi JA, Hughson AR, Horvath AP, Lamparelli AC, Holl KL, Chitre AS, Polesskaya O, Ishiwari K, Solberg Woods LC, Palmer AA, Robinson TE, Flagel SB, & Meyer PJ. Senstivity to food and cocaine cues are independent traits in a large sample of heterogeneous stock rats. Scientific Reports, 11, 2223. https://doi.org/10.1038/s41598-020-80798-w
Sensitivity to cocaine and its associated stimuli (“cues”) are important factors in the development and maintenance of addiction. Rodent studies suggest that this sensitivity is related, in part, to the propensity to attribute incentive salience to food cues, which, in turn, contributes to the maintenance of cocaine self-administration, and cue-induced relapse of drug-seeking. Whereas each of these traits has established links to drug use, the relatedness between the individual traits themselves has not been well characterized in preclinical models. To this end, the propensity to attribute incentive salience to a food cue was first assessed in two distinct cohorts of 2716 outbred heterogeneous stock rats (HS; formerly N:NIH). We then determined whether each cohort was associated with performance in one of two paradigms (cocaine conditioned cue preference and cocaine contextual conditioning). These measure the unconditioned locomotor effects of cocaine, as well as conditioned approach and the locomotor response to a cocaine-paired floor or context. There was large individual variability and sex differences among all traits, but they were largely independent of one another in both males and females. These findings suggest that these traits may contribute to drug-use via independent underlying neuropsychological processes.